24-hour Urine Collection

This test helps us understand how your body is working over the course of a full day. It gives us important information about kidney function and protein levels, which can guide safe and thoughtful care for you and your baby. It may feel a little inconvenient, but taking your time and following the steps carefully will give us the most accurate results.

What You’ll Need

• Your collection container

• A large cup for collecting urine. (Some people use a red-solo cup)

• A place in the refrigerator or a cooler with ice to store the urine during collection

Step-by-Step Instructions

1. Choose Your Start Time

• Pick a time that works well for you (morning is often easiest)

2. Empty Your Bladder — Do NOT Save This First Urine

• At your start time, urinate into the toilet

• Do not collect this urine

• Write down this time — this is your official start time

3. Collect ALL Urine for the Next 24 Hours

• Every time you urinate after that, collect it

• Pour it into the provided container

• Do not miss any urine during this time

4. Store Properly

• Keep the container refrigerated or on ice between collections

• This keeps the sample stable and accurate

5. Finish at the Same Time the Next Day

• At the exact same time you started (24 hours later), urinate one final time

• Collect this last urine and add it to the container

Important Tips

• Try not to spill or miss any collections — accuracy matters

• If you accidentally miss a sample, call us for guidance

• Stay hydrated as usual (don’t over- or under-drink)

• Keep the lid tightly closed

After You Finish

• Return the container to our office. Text Margie at 469-643-9433 when you’re heading in so she can prepare the sample and send it to Quest.

• Try to return it as soon as possible after completing the test

When to Call Us

• If you feel unsure about any step

• If your symptoms change or worsen